Human tryptophan transfer ribonucleic acid synthetase. Composition, function of thiol groups, and structure of thiol peptides.
نویسندگان
چکیده
منابع مشابه
Human tryptophan transfer ribonucleic acid synthetase. Composition, function of thiol groups, and structure of thiol peptides.
Human tryptophanyl-tRNA synthetase resembles its counterpart in Escherichia coli in quaternary structure (alpha2), but differs in molecular weight, amino acid composition, the number of thiol groups, and the relationship of the thiol groups to enzyme activity. Nevertheless, one of the thiol groups resides in a heptapeptide sequence homologous to a heptapeptide sequence containing a thiol group ...
متن کاملTryptophanyl transfer ribonucleic acid synthetase of Escherichia coli. Character of required thiol group and structure of thiol peptides.
Native tryptophanyl-tRNA synthetase purified from Escherichia coli B has on each identical subunit a single thiol group which rapidly forms a mixed disulfide with a thionitrobenzoate moiety of 5,5'-dithiobis(2-nitrobenzoic acid). The reaction and the concomitant inactivation of the enzyme are both reversible by reductive removal of the thionitrobenzoate with dithiothreitol. Iodoacetamide and N-...
متن کاملStructure and function of transfer ribonucleic acid. 3. Some properties of a complex between valyl transfer ribonucleic acid synthetase and transfer ribonucleic acid specific for valine.
The formation and some properties of a stable complex between valyl transfer ribonucleic acid synthetase and transfer RNA from yeast have been investigated by three different methods for the isolation of the complex: titration over Sephadex, sucrose gradient centrifugation, and electrophoresis on Pevikon as supporting medium. Transfer RNA specific for valine (tRNAvsl) can be separated into two ...
متن کاملStructure and function of transfer ribonucleic acid. IV. Complexes between valyl transfer ribonucleic acid synthetase and structurally modified transfer ribonucleic acid specific for valine.
The formation of a stable complex between valyl transfer ribonucleic acid synthetase from yeast and transfer RNA specific for valine (tRNAVa’) from the same source has been used as a model for the recognition between enzyme and tRNA. With the use of sucrose gradient centrifugation to isolate the complex, the major fraction of tRNAVal (tRNAp’) was found to retain its ability to form a stable com...
متن کاملAffinity chromatography of thiol-containing purines and ribonucleic acid.
Evidence is presented for the specific interaction of 6-mercaptopurine with mercurated cellulose. Following from this, a new method is described for the affinity chromatography of thiol-containing molecules and of RNA containing incorporated 6-thioguanosine on columns of mercurated cellulose. This technique may find application in the study of RNA metabolism and gene expression.
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1976
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)33432-4